uMetaP: ultra-sensitive metaproteomics for the dark metaproteome
High-sensitivity LC-MS and FDR-validated de novo sequencing expand detection of low-abundance microbial and host proteins.
Nature Communications
uMetaP lowers detection limits for low-abundance proteins in complex microbial samples by combining sensitive mass spectrometry with de novo sequencing, enabling host–microbiome functional-network analysis.
Metaproteomics projects should retain database versions, FDR settings, contamination screening and missing-value handling.