GENOMICS WORKFLOWS

Genomics

A traceable path from sample to result across extraction, amplification, sequencing preparation and quality control.

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WORKFLOW MAP

From sample to result

01

Nucleic-acid extraction

Control inhibitors and preserve nucleic-acid integrity through lysis, binding, washing and elution.

  1. Sample lysis
  2. Magnetic-bead or membrane binding
  3. Inhibitor removal
  4. Low-volume elution

RisksInhibitors, low-abundance samples, carryover and recovery variation.

QCConcentration, purity, integrity and negative controls.

Recommended products

核酸提取仪 BFEX-32 磁珠法组织基因组DNA提取纯化试剂盒 BFMP01M 核酸提取或纯化试剂 BFMP02R 核酸提取仪 BFEX-96 磁珠法组织基因组DNA提取纯化试剂盒(预分装) BFMP01R 核酸提取或纯化试剂 BFMP02R96 核酸提取仪 BFEX-96E 磁珠法组织基因组DNA提取纯化试剂盒(预分装) BFMP01R1 核酸提取或纯化试剂 BFMP04R 核酸提取仪 BFEX-24E 磁珠法组织基因组DNA提取纯化试剂盒(预分装) BFMP01R96 核酸提取或纯化试剂 BFMP04R96 核酸提取仪 BFEX-32E 磁珠法斑马鱼鳍组织基因组DNA提取纯化试剂盒(预分装) BFMP01RD 核酸提取或纯化试剂 BFMP05R 核酸提取仪 BFEX-4E 磁珠法全血基因组DNA提取纯化试剂盒(预分装) BFMP02R 核酸提取或纯化试剂 BFMP05R96 核酸提取仪 BFEX-16E 磁珠法全血基因组DNA提取纯化试剂盒(预分装) BFMP02R1 核酸提取或纯化试剂 BFMP06R 全自动核酸提取纯化仪 BFEX-32 磁珠法全血基因组DNA提取纯化试剂盒(预分装) BFMP02R96 核酸提取或纯化试剂 BFMP06R96 全自动核酸提取纯化仪 BFEX-96 磁珠法植物基因组DNA提取纯化试剂盒(预分装板) BFMP03R 核酸提取或纯化试剂 BFMP08L 全自动核酸提取纯化仪 BFEX-96E 磁珠法植物基因组DNA提取纯化试剂盒(预分装板) BFMP03R1 核酸提取或纯化试剂 BFMP08R1 全自动核酸提取纯化仪 BFEX-32E 磁珠法植物基因组DNA提取纯化试剂盒(预分装板) BFMP03R96 核酸提取或纯化试剂 BFMP08R 全自动核酸提取纯化仪 BFEX-24E 陈皮基因组DNA纯化试剂盒(离心柱法) BFMP03C 核酸提取或纯化试剂 BFMP08R96 磁珠法病毒基因组DNA提取纯化试剂盒(预分装板) BFMP04R 核酸提取或纯化试剂 BFMP10R 磁珠法病毒基因组DNA提取纯化试剂盒(预分装板) BFMP04R1 核酸提取或纯化试剂 BFMP10R96 磁珠法病毒基因组DNA提取纯化试剂盒(预分装板) BFMP04R96 核酸提取或纯化试剂 BFMP11R24 磁珠法干血斑基因组DNA提取纯化试剂盒(预分装板) BFMP05R 核酸提取或纯化试剂 BFMP11R 磁珠法干血斑基因组DNA提取纯化试剂盒(预分装板) BFMP05R1 核酸提取或纯化试剂 BFMP12R 磁珠法干血斑基因组DNA提取纯化试剂盒(预分装板) BFMP05R96 核酸提取或纯化试剂 BFMP12R96 磁珠法口腔拭子基因组DNA 提取纯化试剂盒(预分装板) BFMP06R 核酸提取或纯化试剂 BFMP14R 磁珠法口腔拭子基因组DNA 提取纯化试剂盒(预分装板) BFMP06R1 核酸提取或纯化试剂 BFMP14R96 磁珠法口腔拭子基因组DNA 提取纯化试剂盒(预分装板) BFMP06R96 核酸提取或纯化试剂 BFMP15R 磁珠法总RNA提取纯化试剂盒(预分装板) BFMP07R 核酸提取或纯化试剂 BFMP15R96 磁珠法总RNA提取纯化试剂盒(预分装板) BFMP07R1 核酸提取或纯化试剂 BFMP25R 磁珠法总RNA提取纯化试剂盒(预分装板 BFMP07R96 核酸提取或纯化试剂 BFMP25R96 磁珠法总RNA提取纯化试剂盒(预分装板)去DNA版 BFMP07R-A 磁珠法总RNA提取纯化试剂盒(预分装板)去DNA版 BFMP07R1-A 磁珠法病毒DNA/RNA提取纯化试剂盒(预分装板) BFMP08R16 磁珠法病毒DNA/RNA提取纯化试剂盒(预分装板) BFMP08L 磁珠法病毒DNA/RNA提取纯化试剂盒(预分装板) BFMP08R 磁珠法病毒DNA/RNA提取纯化试剂盒(预分装板) BFMP08R1 磁珠法病毒DNA/RNA提取纯化试剂盒(预分装板) BFMP08R96 磁珠法质粒DNA提取纯化试剂盒(预分装板) BFMP09R 磁珠法质粒DNA提取纯化试剂盒(预分装板) BFMP09R1 磁珠法质粒DNA提取纯化试剂盒(预分装板) BFMP09R96 磁珠法细菌基因组DNA提取纯化试剂盒(预分装板) BFMP10R 磁珠法细菌基因组DNA提取纯化试剂盒(预分装板) BFMP10R1 磁珠法细菌基因组DNA提取纯化试剂盒(预分装板) BFMP10R96 磁珠法血浆游离DNA提取纯化试剂盒(预分装板) BFMP11R24 磁珠法血浆游离DNA提取纯化试剂盒(预分装板) BFMP11R 磁珠法石蜡包埋组织基因组DNA提取纯化试剂盒(预分装) BFMP12R 磁珠法石蜡包埋组织基因组DNA提取纯化试剂盒(预分装) BFMP12R1 磁珠法石蜡包埋组织基因组DNA提取纯化试剂盒(预分装) BFMP12R96 磁珠法病毒基因组RNA提取纯化试剂盒(预分装板) BFMP14R 磁珠法病毒基因组RNA提取纯化试剂盒(预分装板) BFM14R1 磁珠法病毒基因组RNA提取纯化试剂盒(预分装板) BFMP014R96 磁珠法粪便基因组DNA提取纯化试剂盒(预分装) BFMP15R 磁珠法粪便基因组DNA提取纯化试剂盒(预分装) BFMP15R1 磁珠法粪便基因组DNA提取纯化试剂盒(预分装) BFMP15R96 磁珠法植物RNA提取纯化试剂盒(预分装) BFMP16R 磁珠法植物RNA提取纯化试剂盒(预分装) BFMP16R1 磁珠法植物RNA提取纯化试剂盒(预分装) BFMP16R96 磁珠法植物RNA提取纯化试剂盒(预分装)去DNA版 BFMP16R-A 磁珠法植物RNA提取纯化试剂盒(预分装)去DNA版 BFMP16R1-A 磁珠法环境样本基因组DNA提取纯化试剂盒(预分装) BFMP17R 磁珠法环境样本基因组DNA提取纯化试剂盒(预分装) BFMP17R1 磁珠法环境样本基因组DNA提取纯化试剂盒(预分装) BFMP17R96 磁珠法水生动物基因组DNA提取纯化试剂盒(预分装) BFMP21R 磁珠法水生动物基因组DNA提取纯化试剂盒(预分装) BFMP21R1 磁珠法水生动物基因组DNA提取纯化试剂盒(预分装) BFMP21R96 磁珠法水稻基因组DNA提取纯化试盒(预分装) BFMP23R 磁珠法水稻基因组DNA提取纯化试盒(预分装) BFMP23R96 磁珠法病毒DNA/RNA提取纯化试剂盒(Ultra)(预分装) BFMP25R 磁珠法病毒DNA/RNA提取纯化试剂盒(Ultra)(预分装) BFMP25R1 磁珠法病毒DNA/RNA提取纯化试剂盒(Ultra)(预分装) BFMP25R96 磁珠法细菌RNA提取纯化试剂盒(预分装) BFMP26R 磁珠法细菌RNA提取纯化试剂盒(预分装) BFMP26R1 磁珠法细菌RNA提取纯化试剂盒(预分装) BFMP26R96
Automatic nucleic-acid extractorsResearch nucleic-acid extraction reagentsMedical nucleic-acid extraction reagents Configure this workflow
02

Amplification and detection

Use primer design, reaction composition, cycling parameters and controls to support reliable PCR detection.

  1. Primer and probe design
  2. Reaction setup
  3. Cycling optimization
  4. Positive and negative controls

RisksAmplification bias, non-specific products, contamination and poor templates.

QCCt or endpoint signal, melt curve, repeatability and control results.

Recommended products

QuantFinder定量PCR分析系统 BFQP-1650 自动化基因扩增仪主机 MFCC-A QuantFinder定量PCR分析系统 BFQP-4850 自动化基因扩增仪模块 MFC-96A QuantFinder定量PCR分析系统 BFQP-96 自动化基因扩增仪模块 MFC-96B QuantFinder定量PCR分析系统 BFQP-9650 FastCycler基因扩增仪 FC-96GE QuantFinder定量PCR分析系统 BFQP-9650 FastCycler基因扩增仪 FC-1536G QuantFinder定量PCR分析系统 BFQP-96 FastCycler基因扩增仪 FC-384G QuantFinder定量PCR分析系统 BFQP-96X FastCycler基因扩增仪 FC-96B 实时荧光定量PCR分析仪 BFQP-4850 FastCycler基因扩增仪 FC-96B FastCycler基因扩增仪 FC-96BE FastCycler基因扩增仪 FC-48D FastCycler基因扩增仪 FC-48D
Qualitative PCR amplifiersReal-time qPCR systems Configure this workflow
03

Sequencing library preparation

Coordinate fragmentation, end repair, adapter ligation, indexing, enrichment and library quantification.

  1. Input DNA/RNA review
  2. Fragmentation and end repair
  3. Adapter ligation and indexing
  4. Enrichment and quantification

RisksInsufficient input, fragment bias, adapter dimers and PCR bias.

QCLibrary concentration, fragment distribution, molarity and negative controls.

Recommended products

解脲脲原体（UU）核酸检测试剂盒（PCR荧光探针法） BFRT111M 96孔2.2mL深孔板 BFMH01A 沙眼衣原体（CT）核酸检测试剂盒（PCR荧光探针法） BFRT112M 6孔2.2ml深孔板 BFMH01B 淋球菌（NG）核酸检测试剂盒（PCR荧光探针法） BFRT113M 磁棒套（八联） BFMH02 乙型肝炎病毒（HBV）核酸定量检测试剂盒（PCR荧光探针法） BFRT114M 磁棒套（八联） BFMH02B 乙型肝炎病毒（HBV）核酸定量检测试剂盒（PCR荧光探针法） BFRT114D6 磁棒套（八联） BFMH02T 丙型肝炎病毒（HCV）核酸定量检测试剂盒（PCR荧光探针法） BFRT115M 96孔2.2mL深孔板 BFMH07 丙型肝炎病毒（HCV）核酸定量检测试剂盒（PCR荧光探针法） BFRT115D6 磁棒套（96联） BFMH08 人类ALDH2基因多态性核酸检测试剂盒（PCR荧光探针法） BFRT116M 磁棒套（96联） BFMH08E 人类CYP2C9基因多态性核酸检测试剂盒（PCR荧光探针法） BFRT117M 单样本单支底座固定套 BFMH10 10ul吸头 BFMH12 200ul吸头 BFMH13 1000ul吸头 BFMH14 100ul吸头 BFMH21 八联PCR管 BFMH15 八联PCR管 BFMH15B 24孔15ml深孔板 BFMH16 磁棒套（15ml） BFMH17 24孔10ml深孔板 BFMH18 磁棒套（10ml） BFMH19 研磨管 BFMH20-1 研磨管 BFMH20-2 研磨管 BFMH20-3 研磨管 BFMH20-4
Supporting consumablesResearch diagnostic reagents Configure this workflow
04

Quality control

Combine concentration, purity, fragment distribution, contamination and coverage in one QC record.

  1. Concentration and purity
  2. Fragment distribution
  3. Contamination screening
  4. Coverage and signal quality

RisksOutdated references, inconsistent thresholds, sample mix-up and missing parameters.

QCRetain instrument, reagent, platform, reference-version and action logs.

Recommended products

电泳仪电源 BFEP-300 凝胶成像系统 BFGI-500 超微量分光光度计 BFMUV-3100 miniDNA迷你水平电泳槽 BFEB-100 凝胶成像系统 BFGI-600 超微量分光光度计 BFMUV-3100F midiDNA多用途水平电泳槽 BFEB-200 电泳仪电源 BFEP-600 垂直槽 BFEB-PR04 转印槽 BFEB-BLOT4
Gel imaging systemsElectrophoresis instruments and power suppliesUltramicro spectrophotometers Configure this workflow

Research evidence is provided for workflow context and is not a product-performance guarantee.

RESEARCH UPDATES

Recent research and workflow evidence